This webcast demonstrates that field-flow fractionation (FFF) interfaced with multi-angle light scattering (MALS) and other detectors is an ideal tool for characterization of different drug delivery systems, including liposomes and adeno-associated viruses.

In particular, we will discuss how:

• Properties like size and surface charge of liposomal doxorubicin can be measured by electrical asymmetrical flow FFF interfaced with a MALS detector in the course of few runs

• Different serotypes of adeno-associated viruses (AAV) and their aggregation behavior when they are exposed to heat can be studied using asymmetrical flow FFF-MALS

• Centrifugal FFF can be used to measure the mass difference between empty and filled liposomes

• Centrifugal FFF coupled to MALS can measure liposome density by measuring its mass and size simultaneously in a single run


Key Learning Objective:

• How electrical asymmetrical flow FFF works, and how it can reveal information about size and surface charge of liposomal drug delivery systems

• How different AAV serotypes aggregate when they are exposed to heat, and how flow FFF can separate aggregates and fragments from monomers

• How centrifugal FFF works, and how it can measure masses of empty and drug-loaded liposomes


A short introduction to Postnova’s Field-Flow Fractionation (FFF) platform. This includes: Asymmetric Flow FFF (AF4) which separates by size, Electrical AF4 (EAF4) which separates by size and charge, Centrifugal FFF (CF3) which separates by mass, and Thermal FFF (TF3) which separates by molecular weight and chemical composition. The size exclusion chromatography (SEC) option provides possibilities for orthogonal data. Detectors for FFF and SEC include refractive index, UV/Vis, multi-angle light scattering, dynamic light scattering, viscometry, inductively coupled plasma mass spectrometry, and more.

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A short introduction to the principle and applications of multi-angle light scattering from Postnova Analytics. The combination of having 21 scattering angles, more low scattering angles than any other detector, and the Clean By Design cell, results in superior sensitivity, precision, accuracy, and robustness.



Postnova Analytics’ Founder and CEO, Thorsten Klein, discusses the versatility of Field-Flow Fractionation (FFF). This technique, which uses an open flow channel to separate particles and molecules larger than what can be achieved with column chromatography, can be coupled with a wide variety of detectors. These detectors include multi-angle light scattering, dynamic light scattering, and inductively coupled plasma mass spectrometry, making FFF a very powerful separation technique. As a result, it is used for a variety of applications in biology, chemistry, nanomedicine, and more.

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