Postnova Analytics reports on how Thermal Field Flow Fractionation has been successfully used to elucidate the core microstructure, morphology and chain arrangement of self-assembling block copolymers. This pioneering study was undertaken by the renowned research group under Professor H Pasch at the Department of Chemistry and Polymer Science, University of Stellenbosch (South Africa).

Postnova Analytics reports on how Asymmetrical Flow Field-Flow Fractionation coupled to Multi Angle Light Scattering (AF4-MALS) has been successfully used to characterise a polydisperse cellulose nanocrystal for size and thickness in just 40 minutes analysis time.

Nanoparticles including liposomes are increasingly used for delivery of drug molecules. During formulation of a liposome around a drug particle, a relatively large amount of free drug may remain unencapsulated and therefore not available for drug delivery via the liposomal carrier. For formulation groups adopting this drug delivery methodology it is important to quantify the amount of free versus encapsulated drug in a product in order to accurately determine the delivered dose of drug to a patient.

Postnova Analytics reports on a recent independent review** which details how Asymmetric Flow Field-Flow Fractionation (AF4) is a valuable technique to improve the quality of nanoformulated medicines.

Currently, only a few standardized methods exist to characterize some of the crucial physicochemical properties and their impact on biological systems of medical nanoparticles. Unfortunately, they are often not applicable to the more complex and innovative nanoformulations. 

Particle size and size distribution are key factors for the manufacturing quality, as well as the efficacy and safety of medicinal nanoparticles. Despite its widespread usage in the nanomedicine field, batch-mode dynamic light Scattering (DLS) is generally only considered a ‘low-resolution’ method for checking the integrity of monodispersed samples. To overcome the inherent difficulties that are imposed when analysing a polydisperse nanoformulated sample by DLS, a fractionation step should be incorporated prior to measurement. This can be done by using DLS and MALS detectors coupled online to a Postnova Analytics Asymmetric Flow Field Flow Fractionation (AF4) system.

The PN3621 Multi-Angle Light Scattering (MALS) detector from Postnova Analytics combines outstanding performance, high precision and reliability design features to facilitate advanced characterization of proteins, conjugates, macromolecules, and nanoparticles.

The PN3621 is commonly used in conjunction with Gel Permeation Chromatography (GPC-MALS), Size Exclusion Chromatography (SEC-MALS) or Field-Flow Fractionation (FFF-MALS) to determine distributions of mass, size and composition independent of column calibration by reference standards.

Designed for many years of reliable, trouble-free operation the customer-friendly PN3621 incorporates a unique vertical ‘clean by design’ flow cell. Unlike MALS instruments with problem prone horizontal flow cells, the vertical cell design 'self-cleans' and also enables large and potentially fragile high molar mass macromolecules to pass through unaltered.

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