Postnova Analytics reports on an informative new book that introduces scientists to Thermal Field-Flow Fractionation and discusses its advantages over the fundamental problems associated with traditional column-based analytical techniques commonly used to characterize polymers and macromolecules.

The PN3621 Multi-Angle Light Scattering (MALS) detector from Postnova Analytics combines outstanding performance, high precision and reliability design features to facilitate advanced characterization of proteins, conjugates, macromolecules, and nanoparticles. The PN3621 is commonly used in conjunction with Gel Permeation Chromatography (GPC-MALS), Size Exclusion Chromatography (SEC-MALS) or Field-Flow Fractionation (FFF-MALS) to determine distributions of mass, size and composition independent of column calibration by reference standards.

Nanoparticles including liposomes are increasingly used for delivery of drug molecules. During formulation of a liposome around a drug particle, a relatively large amount of free drug may remain unencapsulated and therefore not available for drug delivery via the liposomal carrier. For formulation groups adopting this drug delivery methodology it is important to quantify the amount of free versus encapsulated drug in a product in order to accurately determine the delivered dose of drug to a patient.

Postnova Analytics reports on how Asymmetrical Flow Field-Flow Fractionation coupled to Multi Angle Light Scattering (AF4-MALS) has been successfully used to characterise a polydisperse cellulose nanocrystal for size and thickness in just 40 minutes analysis time.