Knowledge >Scientific Papers >Scientific Paper

Scientific Paper

Application of flow field-flow fractionation for the characterization of macromolecules of biological interest: a review

Reference Type:Journal Article
Author:Qureshi, Rashid;Kok, Wim
Author Addresses:Analytical Chemistry Group, van ’t Hoff Institute for Molecular Sciences, University of Amsterdam, PO Box 94157, 1090 GD Amsterdam, The Netherlands
Title:Application of flow field-flow fractionation for the characterization of macromolecules of biological interest: a review
Secondary Title:Analytical and Bioanalytical Chemistry
Periodical Title:Analytical and Bioanalytical Chemistry
Pages:1401-1411
Volume:399
Number:4
Keywords:Flow field-flow fractionation,Biomacromolecules,Bioparticles,Molecular weight distribution,Particle size distribution
Year:2011
Publishing Date: 20.10.2010
ISBN:1618-2642
Related URLs:http://dx.doi.org/10.1007/s00216-010-4278-3

An overview is given of the recent literature on (bio) analytical applications of flow field-flow fractionation (FlFFF). FlFFF is a liquid-phase separation technique that can separate macromolecules and particles according to size. The technique is increasingly used on a routine basis in a variety of application fields. In food analysis, FlFFF is applied to determine the molecular size distribution of starches and modified celluloses, or to study protein aggregation during food processing. In industrial analysis, it is applied for the characterization of polysaccharides that are used as thickeners and dispersing agents. In pharmaceutical and biomedical laboratories, FlFFF is used to monitor the refolding of recombinant proteins, to detect aggregates of antibodies, or to determine the size distribution of drug carrier particles. In environmental studies, FlFFF is used to characterize natural colloids in water streams, and especially to study trace metal distributions over colloidal particles. In this review, first a short discussion of the state of the art in instrumentation is given. Developments in the coupling of FlFFF to various detection modes are then highlighted. Finally, application studies are discussed and ordered according to the type of (bio) macromolecules or bioparticles that are fractionated.

Sitemap