Postnova Analytics reports on an informative new book** that introduces scientists to Thermal Field-Flow Fractionation and discusses its advantages over the fundamental problems associated with traditional column-based analytical techniques commonly used to characterize polymers and macromolecules.
Thermal Field-Flow Fractionation (TF3) is an exciting technique that provides a highly efficient method of separating and characterizing complex polymer samples such as natural or synthetic rubbers, starches and paints from approximately 5 kDa up to 1000 MDa and more in both organic and aqueous solvents. The TF3 technique uses a gentle temperature gradient as the driving force for its separation of polymers and particles, meaning there is no packing material present to interact with the sample and no high shear forces.
Molecules affected by the thermal gradient undergo diffusion which enables separation by both their molar mass and chemical composition. This unique feature allows the separation of different materials having the same molar mass. The separation can be further optimized by the use of different eluents and various temperature programs.
Read more … New Book Thermal Field-Flow Fractionation of Polymers
The PN3621 Multi-Angle Light Scattering (MALS) detector from Postnova Analytics combines outstanding performance, high precision and reliability design features to facilitate advanced characterization of proteins, conjugates, macromolecules, and nanoparticles.
The PN3621 is commonly used in conjunction with Gel Permeation Chromatography (GPC-MALS), Size Exclusion Chromatography (SEC-MALS) or Field-Flow Fractionation (FFF-MALS) to determine distributions of mass, size and composition independent of column calibration by reference standards.
Designed for many years of reliable, trouble-free operation the customer-friendly PN3621 incorporates a unique vertical ‘clean by design’ flow cell. Unlike MALS instruments with problem prone horizontal flow cells, the vertical cell design 'self-cleans' and also enables large and potentially fragile high molar mass macromolecules to pass through unaltered.
Read more … The PN3621 Multi-Angle Light Scattering (MALS) detector from Postnova Analytics
Postnova Analytics reports on a recent independent review** which details how Asymmetric Flow Field-Flow Fractionation (AF4) is a valuable technique to improve the quality of nanoformulated medicines.
Currently, only a few standardized methods exist to characterize some of the crucial physicochemical properties and their impact on biological systems of medical nanoparticles. Unfortunately, they are often not applicable to the more complex and innovative nanoformulations.
Particle size and size distribution are key factors for the manufacturing quality, as well as the efficacy and safety of medicinal nanoparticles. Despite its widespread usage in the nanomedicine field, batch-mode dynamic light Scattering (DLS) is generally only considered a ‘low-resolution’ method for checking the integrity of monodispersed samples. To overcome the inherent difficulties that are imposed when analysing a polydisperse nanoformulated sample by DLS, a fractionation step should be incorporated prior to measurement. This can be done by using DLS and MALS detectors coupled online to a Postnova Analytics Asymmetric Flow Field Flow Fractionation (AF4) system.
Read more … Field-Flow Fractionation Improves Characterization of Nanoformulated Medicines
Nanoparticles including liposomes are increasingly used for delivery of drug molecules. During formulation of a liposome around a drug particle, a relatively large amount of free drug may remain unencapsulated and therefore not available for drug delivery via the liposomal carrier. For formulation groups adopting this drug delivery methodology it is important to quantify the amount of free versus encapsulated drug in a product in order to accurately determine the delivered dose of drug to a patient.
Read more … Technique for Characterising Liposome Formulated Drugs
Postnova Analytics reports on how Asymmetrical Flow Field-Flow Fractionation coupled to Multi Angle Light Scattering (AF4-MALS) has been successfully used to characterise a polydisperse cellulose nanocrystal for size and thickness in just 40 minutes analysis time.
Read more … Fast Measurement of Nanocrystal Length & Thickness
Postnova Analytics reports on how Thermal Field Flow Fractionation has been successfully used to elucidate the core microstructure, morphology and chain arrangement of self-assembling block copolymers. This pioneering study was undertaken by the renowned research group under Professor H Pasch at the Department of Chemistry and Polymer Science, University of Stellenbosch (South Africa).
Read more … Separation & Characterisation of Self Assembling Block Copolymers
Postnova Analytics has published a new application note that describes a new approach for analysis of titanium dioxide nanoparticles in commercial sunscreens. The technique, which combines Inverse Supercritical Fluid Extraction (I-SFE) and Miniaturized Asymmetrical Flow Field-Flow Fractionation hyphenated with UV-Vis and Multi Angle Light Scattering (mAF4-UV-MALS) represents a promising tool for the verification of the nano-labelling of such products.
Read more … Analysis of Nanoparticles in Commercial Sunscreens
Postnova Analytics has published a new poster that compares the suitability of Electrical Asymmetrical Flow Field-Flow Fractionation (EAF4) and Size Exclusion Chromatography (SEC) for determining the physicochemical and biophysical attributes of monoclonal antibodies.
Read more … Comparative Analysis of a Complex Monoclonal Antibody
Postnova Analytics announce in a joint collaboration with the Department of Biosciences at the University of Helsinki (Finland), that Asymmetrical Flow Field-Flow Fractionation (AF4) was successfully employed for the purification of halophilic (extreme) viruses.
Read more … Efficient Purification of Extreme Viruses
Postnova Analytics report on how researchers from the Institute for Health and Consumer Protection at the European Commission Joint Research Centre (Ispra, Italy) and the University of Ferrara have used AF2000 Asymmetric Flow Field-Flow Fractionation (AF4) system and Model S101 Sedimentation Flow-Field Fractionation (SdFFF) systems in some ground-breaking research.
Read more … Comparing Methods to Detect, Size and Quantify Silver Nanoparticles
Postnova Analytics reports on how the SWAMP ultra-clean research facility* at the University of Alberta (Alberta, Canada) has been using the AF2000 Flow field flow fractionation system coupled to Inductively Coupled Plasma Mass Spectrometry (ICP-MS), UV and fluorescence detectors to characterise trace metal colloid distribution in the environment.
Read more … Measuring Trace Metal Colloid Distribution in The Environment
Postnova Analytics reports on how the inorganic analysis team within LGC (Teddington, UK) has been using the AF2000 Field Flow Fractionation system coupled to Inductively Coupled Plasma Mass Spectrometry (ICP-MS) to characterise nanomaterials in complex food sample matrices.
Read more … Characterisation of Nanomaterials in Foods
Postnova Analytics reports that the Department of Molecular Biology & Biotechnology at the University of Sheffield (UK) is using their AF2000 Field Flow Fractionation system to investigate the mechanism of amyloid formation, a protein mis-folding process implicated to be the cause of Alzheimer’s and other neurodegenerative diseases.
Read more … Unlocking the Puzzle of Neurodegenerative Diseases