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AF2000 MultiFlow FFF - Universal Separator


AF2000 MT - Mid Temperature Flow FFF

The most sophisticated Advanced-Level Flow FFF system for Research and QC Applications.


Application Range

The AF2000 MT is the modular Advanced-Level system of the Postnova AF2000 MultiFlow platform. The system includes sophisiticated temperature control and runs at conditions from 5° to 90°C. This makes the system even more stable due to the temperature stabilized diffusion process. Also separations of fragile biomolecules at lower temperatures as well as high molar mass macromolecules requiring higher temperatures are possible. The AF2000 MT therefore offers the highest reproducibility and the maximum application range within the AF2000 MultiFlow Platform. The system is ideal for special research applications in university, public and industrial research groups and also for high demanding QC applications in production environments. The AF2000 MT is the best choice for advanced FFF groups which want to work at the highest technical level and expect maximum performance from their FFF. The system is available as aqueous metal-free, stainless steel and multisolvent version and can be run with a broad range of aqueous and organic solvents. Planar Asymmetric Flow FFF (AF4) and circular HollowFiber Flow FFF (HF5) channels are standard and can be used at the same time. Various detection principles, such as UV-Absorbance (UV), Refractive Index (RI), Multi-Angle Light Scattering (MALS), Dynamic Light Scattering (DLS) and Inductively Coupled Plasma Mass Spectroscopy (ICP-MS) can be coupled easily online.

System Hardware

It is Postnova's clear mission to only offer fully optimized and professional FFF hardware, software and support solutions. Therefore the AF2000 MT series is a completely optimized Flow FFF system, which contains all necessary standard modules and components in the package, that are required for a robust, reliable and stable operable Flow FFF system. There is no need for assembling and utilizing semi-suited third party HPLC components, which are often just made for other purposes, show less flexibility and are not available in the strict specifications needed for FFF applications. The AF2000 MT series is a true ready-to-use non-compromise Flow FFF system, where no additional components are required.

The system incorporates the complete set of Postnova's proven FFF technologies, such as FocusTech, ChannelCartridge and SyringeXFlow, which make it the most easy-to-use, robust and reproducible system available. The AF2000 MT system is comprising an Eluent Organizer (PN7205), an Eluent Degasser (PN7520), an Eluent Pump (PN1130), a Focus Pump (PN1130), a Flow FFF Control Module with integrated electronic interface and cross-flow syringe pump (AF2000 Module), a FFF Channel Thermostat with Manual Injector Holder (PN4020), a user-selectable Asymmetric / HollowFiber Flow FFF channel (AF4/HF5) and a Control PC with the NovaFFF software suite for operation of the AF2000 MT system.

Additional System Options

The AF2000 MT Flow FFF is available in different versions and additionally can be upgraded and customized for special applications by adding extra system options. This can be done at date of purchase or later as an upgrade.

  • EFS Eluent Filtration System
  • UVD Eluent Disinfection Module
  • SAM Manual/Auto Injector with Options
  • ELC Aqu. Metal-free/Organic/MultiSolvent
  • AF4/HF5 Channel Versions and Membranes
  • S3 Outlet Splitting Sensitivity Enhancer
  • RID Refractive Index Detection System
  • UVD Absorbance Detection System
  • FLD Fluorescence Detection System
  • MALS Multi-Angle Light Scattering Detector
  • DLS Zetasizer* Dynamic Light Scattering Detector
  • HCT Multi Heated Capillary Thermostat
  • ICP-MS Online Connection and Interface
  • FRA Automatic Fraction Collector with Options

System Software

The unique NovaFFF single software platform is used for full control and data acquisition of the entire AF2000 MT Flow FFF together with associated peripheric modules (such as Auto Injector, Fraction Collectors) and detectors (LS, RI, UV, FLD). The software also allows comprehensive data analysis and evaluation based on Relative Standard Calibration and Absolute Light Scattering methods. Furthermore the software is also the platform to run all mayor FFF versions, including AF2000 MultiFlow FFF, CF2000 Centrifugal FFF and TF2000 Thermal FFF. This together with additional features, makes NovaFFF the most advanced and leading software platform available for FFF applications today.

Service & Support

In order to assist our users in achieving the best results and getting the maximum output from their FFF system without any time delay, Postnova offers a series of differently adjusted services and support activities. These services have been optimized to perfectly fit each step the live cycle of any FFF system, from installation and initial training, via maintenance during operation all the way to relocation and upgrade services to keep up with new technological developments.

  • Installation Services
  • User Training Courses
  • System Maintenance
  • Relocation Services
  • Upgrade & Update Services

Further customized services and special maintenance contracts are available for the FFF systems including detection and also for some selected modules of the systems. Please inquire at:


Zetasizer* is a trademark of Malvern Instr.

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